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Characterization of ESC-sEVs. A, particle size distribution of ESC-sEVs. B, morphology of USC-Exos under transmission electron microscopy. Scale bar, 200 nm. C, western blots showing the expression of TSG101, CD81, and <t>CALNEXIN</t> in ESC and ESC-sEVs. sEV: small extracellular vesicle; ESC: embryonic stem cell.
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Characterization of ESC-sEVs. A, particle size distribution of ESC-sEVs. B, morphology of USC-Exos under transmission electron microscopy. Scale bar, 200 nm. C, western blots showing the expression of TSG101, CD81, and <t>CALNEXIN</t> in ESC and ESC-sEVs. sEV: small extracellular vesicle; ESC: embryonic stem cell.
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Characterization of ESC-sEVs. A, particle size distribution of ESC-sEVs. B, morphology of USC-Exos under transmission electron microscopy. Scale bar, 200 nm. C, western blots showing the expression of TSG101, CD81, and CALNEXIN in ESC and ESC-sEVs. sEV: small extracellular vesicle; ESC: embryonic stem cell.

Journal: Science Progress

Article Title: Embryonic stem cell-derived small extracellular vesicles alleviate lead-induced cochlear spiral ganglion neurons injury by activating PI3 K/AKT signaling pathway

doi: 10.1177/00368504251358007

Figure Lengend Snippet: Characterization of ESC-sEVs. A, particle size distribution of ESC-sEVs. B, morphology of USC-Exos under transmission electron microscopy. Scale bar, 200 nm. C, western blots showing the expression of TSG101, CD81, and CALNEXIN in ESC and ESC-sEVs. sEV: small extracellular vesicle; ESC: embryonic stem cell.

Article Snippet: The membranes were blocked with TBST containing 5% bovine serum albumin for 2 h and then incubated with TSG101 polyclonal antibody (Proteintech, 282283, 1:1000, CHINA), CD81 monoclonal antibody (Proteintech, 66866, 1:1000, CHINA), CALNEXIN polyclonal antibody (Proteintech, 10427, 1:1000, CHINA), PI3 kinase p110 alpha monoclonal antibody (Proteintech, 670071, 1:1000, CHINA), AKT rabbit polyclonal antibody (Proteintech, 10176, 1:1000, CHINA), phospho-AKT rabbit monoclonal antibody (Proteintech, 66444, 1:1000, CHINA), mTOR rabbit polyclonal antibody (Cell Signaling Technology, 2972S, 1:1000, USA), p-mTOR rabbit polyclonal antibody (Cell Signaling Technology, 2974 T, 1:1000, USA), Bcl-2 rabbit polyclonal antibody (Proteintech, 26593, 1:1000, CHINA), BAX rabbit polyclonal antibody (Proteintech, 50599, 1:1000, CHINA), cleaved caspase-3 rabbit monoclonal antibody (Cell Signaling Technology, 9664 T, 1:1000, USA), or ACTIN mouse monoclonal antibody (Zen Bioscience, 200068, 1:10,000, CHINA) at 4°C overnight.

Techniques: Transmission Assay, Electron Microscopy, Western Blot, Expressing